Journal: Journal of Genetic Engineering & Biotechnology

Article Title: In silico designing of a recombinant multi-epitope antigen for leprosy diagnosis

doi: 10.1186/s43141-022-00411-7

Figure Lengend Snippet: In silico cloning. The recombinant multi-epitope DNA sequence cloned into the pET28a( +) (Addgene) expression vector, represented in red color. The insert was added between the BipI and BamHI restriction sites

Article Snippet: The recombinant multi-epitope DNA sequence cloned into the pET28a( +) (Addgene) expression vector, represented in red color.

Techniques: In Silico, Cloning, Recombinant, Sequencing, Clone Assay, Expressing, Plasmid Preparation

Journal: PLoS ONE

Article Title: Dendritic Cell-Mediated, DNA-Based Vaccination against Hepatitis C Induces the Multi-Epitope-Specific Response of Humanized, HLA Transgenic Mice

doi: 10.1371/journal.pone.0104606

Figure Lengend Snippet: Purified HLA-A2/DRB1 DCs were transfection by electroporation with a GFP-expressing plasmid (NTC8685-eRNA41H-EGFP) and cultured overnight in the presence of GM-CSF to promote viability. The cells were examined visually on the following day: UV microscopy (left), DAPI-counter stain (center), merged images (right).

Article Snippet: The two multi-epitope gene sequences were synthesized by GenScript USA, Inc. (Piscataway, NJ) and cloned into the NTC8685-eRNA41H-EGFP expression vector (Nature Technology Corporation, Lincoln, NE).

Techniques: Purification, Transfection, Electroporation, Expressing, Plasmid Preparation, Cell Culture, Microscopy, Staining